The six minute clip is the second showing the journalist to be circulated by Islamic State (IS) militants in less than a week.
SITE Intelligence Group, which monitors Jihadist groups, described the latest footage as the “first episode” in a series involving Mr Cantlie speaking about the group.
It shows him again sitting at a desk dressed in the now-familiar orange outfit worn by other IS hostages.
Mr Cantlie looks calm and appears to be reciting a prepared script. It does not show any of his captors and features no threats to kill.
The clip is introduced with the title Lend Me Your Ears and Messages From The British Detainee John Cantlie before the Briton appears on screen.
Last week a video surfaced showing Mr Cantlie in a similar setting saying he would speak about IS in future videos.
Both videos appear to be well-produced, with good quality sound and lighting.
Earlier this month, David Haines, 44, who was raised in Perth, was murdered by Islamic State militants. Two US journalists have also been killed following Jihadist videos recently.