New way to help asthma sufferers found

Washington -  A new study has offered new hope to asthma sufferers by identifying ways to reduce the factors that lead to an asthma attack.

A UCSF researcher and his colleagues believe they have found a way to help asthma sufferers by impeding the two most significant biological responses that lead to an asthma attack.

Asthma, a respiratory disorder that causes shortness of breath, coughing and chest discomfort, results from changes in the airways that lead to the lungs.

Researchers from UCSF, Johns Hopkins University and Duke University have demonstrated that a specific calcium-activated chloride channel holds valuable clues to reducing two biological

processes that contribute to the severity of asthma. These channels regulate airway secretions and smooth muscle contraction, the two major factors that lead to an asthma attack.

“Maybe if we could inhibit both of these processes by blocking this one channel, then we could affect the two symptoms of asthma,” said senior author Jason Rock, PhD, assistant professor

at the UCSF Department of Anatomy.

Normally humans have few mucus-producing cells but asthma sufferers have an elevated number of these cells in the lining of the tubes that lead to the lungs. Asthmatics also have an

abnormal amount of smooth muscle surrounding the airway tubes. Even the slightest stimulus can cause these to contract.

“The overabundance of mucus plugging the airways combined with hyper-contractility of the smooth muscle – when the tubes get really small – make it difficult to move air in or out. A lot of

people equate that with breathing through a straw,” Rock said.

Rock and his colleagues focused on a calcium-activated chloride channel called TMEM16A. This channel secretes chloride ions in response to rises in intracellular calcium. It regulates a

significant number of biological processes such as neuron firing, gastrointestinal activity and the secretion of sweat and tears.

The researchers wanted to prove that the channel was present in asthma. They studied human lung samples and looked for the mRNA (messenger RNA which carries information from the

DNA to the sites of protein synthesis), and compared protein levels in asthmatic and non-asthmatic patients.

“We found that the level of this channel was increased in the mucus producing cells of asthmatics compared to non-asthmatics. And we also validated this in several models of asthma,

including mouse models. That was step one,” Rock said.

Step two consisted of a screen to identify chemicals that inhibit the activity of TMEM16A. The authors identified three chemicals that inhibit the channel.

“We tested the ability of these chemicals to inhibit TMEM16A and other channels, and we found that they specifically block TMEM16A. It is great that we came across these molecules that

were unknown previously since we can now try to get into clinical trials to benefit patients,” Rock noted.

Then, the researchers simulated asthma in a dish and blocked the channel to observe whether it affected mucus production.

“Mucus produced by these cells wasn’t secreted as efficiently. We also studied airway smooth muscles of human patients who had given their organs to science, and we could see that

blocking TMEM16A inhibited their contraction response as well,” Rock explained.

Rock is in the pre-clinical phase to test the safety and effectiveness of the blockers in animal studies. If that goes well, Phase One of clinical trials can begin.

A paper on the study has been published in the Proceedings of the National Academy of Sciences (PNAS).